Anti-Human SPP1 Recombinant Antibody (ASK8007)
CAT#: TAB-582CL
Recombinant Humanized monoclonal antibody expressed in CHO binding to Human SPP1. ASK 8007 is an antibody which acts against osteopontin, that has a beneficial effect on joint inflammation in patients with rheumatoid arthritis.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-Human SPP1 Antibody (TAB-582CL) in ELISA
ELISA analysis of TAB-582CL was performed by coating with Human SPP1 Protein (His Tag). Then blocked with BSA and incubated with TAB-582CL. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human SPP1 Antibody (TAB-582CL) in WB
Western blot analysis of TAB-582CL was performed with Human SPP1 Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-582CL (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-Human SPP1 Antibody (TAB-582CL) in DB
Dot Blot analysis of TAB-582CL was performed by coating with Human SPP1 Protein (His Tag).
TAB-582CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
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Subcellular Location
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27540/1789_G12_1_cr59549927dd49e_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/1763_H3_6_selected.jpg
Normal Tissue
Figure 3 Kidney
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_7_5_val_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_8_3.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, Hep G2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118785-SPP1
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Specifications
- Derivation
- Humanized
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Rheumatoid Arthritis
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Target
- Alternative Names
- SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secr
- Gene ID
- 6696
- UniProt ID
- A0A024RDE6
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Enhances Rheumatoid Arthritis Studies
This antibody has been pivotal in examining osteopontin's role in inflammatory pathways, particularly in rheumatoid arthritis. Its specificity and reliable performance in ELISA and WB bolster our confidence in the reproducibility of results, essential for advancing therapeutic research.
Unmatched Specificity and Sensitivity
The sensitivity of this antibody stands out in our ELISA assays for detecting osteopontin levels. It has fundamentally enhanced our study of cellular mechanisms underlying inflammation and provides essential data crucial for our hypothesis testing.
Highly Stable and Robust
The stability of the Anti-SPP1 Antibody during long-term storage has been advantageous for our laboratory, allowing for planned, extended studies without worrying about degradation or activity loss. It supports a rigorous approach to ongoing research challenges.
Q&As
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How does the SPP1 protein contribute to disease processes?
A: Osteopontin (SPP1) is involved in immune regulation and cell signaling pathways applicable to bone resorption, inflammatory conditions, and cancer metastasis. Its expression correlates with aggressive tumor behaviors and inflammatory diseases, marking it a crucial target for therapeutic interventions.
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Is this antibody applicable in various research models?
A: Yes, the antibody's binding efficacy across human and other model systems allows for its use in a range of preclinical studies. This versatility aids in developing and testing therapeutic strategies in a controlled environment before clinical application.
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How stable is the Anti-SPP1 antibody under storage conditions?
A: The antibody is stable when stored at -20°C, and is formulated to withstand multiple freeze-thaw cycles without loss of activity. This stability enhances its utility in longitudinal studies that require repeated use over time.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-582CL, RRID: AB_3111976)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| HPAB-0069-YC | Human Anti-SPP1 Recombinant Antibody (clone c2K1) | FuncS | Chimeric (mouse/human) IgG, κ |
| HPAB-0070-YC | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7) | ELISA | Humanized IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0068-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone h2K1); scFv Fragment | FuncS | Humanized scFv |
| HPAB-0069-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone c2K1); scFv Fragment | FuncS | Mouse scFv |
| HPAB-0070-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7); scFv Fragment | ELISA | Humanized scFv |
| HPAB-1363-FY-F(E) | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12); scFv Fragment | WB, ELISA | Mouse scFv |
| HPAB-0569-YJ-S(P) | Human Anti-SPP1 Recombinant Antibody (clone OPN-3); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0068-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone h2K1); Fab Fragment | FuncS | Humanized Fab |
| HPAB-0069-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone c2K1); Fab Fragment | FuncS | Human Fab |
| HPAB-0070-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1363-FY-S(P) | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12); Fab Fragment | WB, ELISA | Mouse Fab |
| HPAB-1079WJ-F(E) | Human Anti-SPP1 Recombinant Antibody (clone MOR-6990); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY193 | Mouse Anti-SPP1 (clone 23C3) scFv-Fc Chimera | ELISA | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6866 | Anti-SPP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6867 | Anti-Mouse SPP1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-YC228 | Recombinant Anti-SPP1 (Thrombin x MMP cleavage site) Biparatopic Antibody, Tandem scFv (mAb 4-4-2 x mAb 7-5-4) | ELISA, WB | Tandem scFv |
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